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CYP450 Protein Assay – Human Induction Kit AB SCIEX

试剂盒

试剂盒

Protein expression changes of individual cytochrome P450 (CYP450) isoforms can be directly measured using the CYP450 Protein Assay – Human Induction Kits and an LC/mass spectrometry workflow for high specificity, sensitivity, and accuracy. These kits have been developed to employ multiple reaction monitoring (MRM) analysis to quantify seven CYP450 isoforms that are key proteins for induction studies: 1A2, 2B6, 2C9, 2C19, 2E1, 3A4 and 3A5. Kits with varying numbers of isoforms are available: 1) the four-isoform kit (1A2, 2B6, 3A4 and 3A5), and 2) the seven-isoform kit (1A2, 2B6, 2C9, 2C19, 2E1, 3A4 and 3A5).

Part Number Kit Name/Description
4445250 Trypsin TPCK (500 μg)
A modified (TPCK treated) serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Modified trypsin is treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. For use with the P450 Human Induction 100 Assay Kit. Ten tubes are included in each package and each tube will be sufficient for at least 10 samples (1 mg of protein digested with a 1:20 ratio = 50 μg).
4445251 Peptide C18 Column
A reversed phase C18 column specifically designed for the analysis of CYP450 isoforms by LC/MS/MS. Good for at least 200 analyses.
4445252 P450 Human Induction 100 Assay Kit
The P450 Human Induction 100 Assay Kit provides a complete protocol, instrument data acquisition methods, processing methods and stable isotope labeled synthetic peptide standards for the analysis of four human CYP450 isoforms (1A2, 2B6, 3A4, and 3A5) for induction studies using LC/MS/MS on an SCIEX QTRAP® or Triple Quad™ system. Kit contains enough reagents for 100 Assays. Must order Trypsin TPCK (500 ug) and Peptide C18 Column sold separately.
4445494 P450 Human Induction Starter Kit
The P450 Human Induction Starter Kit provides a complete package for the analysis of four human CYP450 isoforms (1A2, 2B6, 3A4, and 3A5) using MRM analysis on an SCIEX QTRAP or Triple Quad system. The kit contains a detailed protocol, instrument data acquisition methods, processing methods, LC column, stable isotope labeled synthetic peptide standards, trypsin, and all buffers and reagents for 100 assays.
4465863 CYP450 Peptide Standards Extended Panel Human Induction 100 Assay Kit
The SCIEX CYP450 Peptide Standards Extended Panel Human Induction 100 Assay Kit provides a complete protocol, instrument data acquisition methods, processing methods and stable isotope labeled synthetic peptide standards for the analysis of seven human CYP450 isoforms (1A2, 2B6, 2C9, 2C19, 2E1, 3A4 and 3A5) for induction studies using LC/MS/MS on an SCIEX QTRAP or Triple Quad system. Kit contains enough reagents for 100 Assays. Trypsin TPCK (500 μg) and Peptide C18 Column sold separately.
4445247 Protein Preparation Kit 100 Assay
Protein preparation kit containing enough reagent for the solubilization, reduction, and alkylation of 100 1-mg protein samples. The kit contains 1 vial of denaturant, 1 vial of reducing reagent, 1 vial of cysteine blocking reagent, and 6 vials of digestion buffer. Trypsin (4445250) sold separately. Note: Not for use with amine labeling reagents – for assays with amine labeling reagents use 4381664.
4466004 CYP450 Peptide Standards Extended Panel Human Induction Starter Kit
The SCIEX CYP450 Peptide Standards Extended Panel Human Induction Starter Kit provides a complete package for the analysis of seven human CYP450 isoforms (1A2, 2B6, 2C9, 2C19, 2E1, 3A4 and 3A5) using MRM analysis on an SCIEX QTRAP or Triple Quad system. The kit contains a detailed protocol, instrument data acquisition methods, processing methods, LC column, stable isotope labeled synthetic peptide standards, trypsin, and all buffers and reagents for 100 assays.