河南东吉高科有限公司
细胞培养
| 小鼠胰岛素瘤胰岛Beta细胞–Beta-TC-6 | DMEM(低碳酸氢钠)+15%FBS+1%P/S 贴壁 |
| 小鼠胰岛内皮细胞-朗格汉斯岛C57BL/6–MS1=Mile Sven 1 | DMEM+10%FBS+1%P/S |
| 小鼠心肌细胞–HL-1 | Claycomb Medium(推荐 Sigma 货号：51800C）+10%FBS+1%P/S 贴壁 |
| 小鼠小胶质细胞–BV2 | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠小肠内分泌细胞–STC-1–ATCC来源全新的种子STR鉴定准确 | DMEM+10%FBS+1%P/S |
| 小鼠视网膜神经节细胞–RGC-5 | F12+10%FBS+1%P/S 贴壁 |
| 小鼠肾足细胞–MPC-5 | DMEM+10%FBS+1%P/S |
| 小鼠肾脏内髓集合管3上皮细胞-肾髓质细胞–IMCD3–ATCC全新种子 | DF12+10%FBS+1%P/S |
| 小鼠肾小球系膜细胞–SV40-MES-13国家库 | DF12+10%FBS+1%P/S 贴壁 |
| 小鼠肾癌细胞–Renca | 1640+10%FBS+1%P/S |
| 小鼠神经干细胞–C17.2 | DMEM+10%FBS+1%P/S |
| 小鼠乳腺上皮细胞–HC11 | 1640+10%FBS+1%P/S |
| 小鼠乳腺癌细胞+LUC–4T1+LUC | 1640+10%FBS+1%P/S 贴壁 |
| 小鼠乳腺癌细胞–4T1 | 1640+10%FBS+1%P/S 贴壁 |
| 小鼠乳腺癌细胞–EMT-6 | DMEM+10%FBS+1%P/S |
| 小鼠乳腺癌高转移细胞–MA-891 | 1640+10%FBS+1%P/S 贴壁 |
| 小鼠前胃癌细胞–MFC | 1640+10%FBS+1%P/S |
| 小鼠前列腺癌细胞–RM-1 | DMEM+10%FBS+1%P/S |
| 小鼠气道平滑肌细胞–ASMC来源不清晰不建议使用 | 1640+10%FBS+1%P/S |
| 小鼠皮下结缔组织细胞–Lwnt-3a | DMEM+10%FBS+1%P/S +G4180.4mg-ml |
| 小鼠胚胎细胞–NIH-3T3 | DMEM+10%（NBS）+1%P/S |
| 小鼠胚胎瘤细胞-软骨细胞–ATDC-5 | DMEM+10%FBS+1%P/S |
| 小鼠胚胎干细胞–E14 | DMEM+15%FBS+0.1uL-mLLIF+1%P/S+1uL-mLβ－巯基乙醇+1%P/S 培养瓶用 0.1%明胶包被细胞呈集落生长 |
| 小鼠胚胎成纤维细胞–3T3-L1 | DMEM(低碳酸氢钠)+10%（NBS）+1%P/S |
| 小鼠胚胎成纤维细胞–BALB-3T3cloneA31 | DMEM+10%FBS+1%P/S 80%密度时1:2传代，生长慢，传代密度要大 |
| 小鼠胚胎成纤维细胞–C3H-10T1-2,Clone8 | MEM+10%FBS+1%P/S 贴壁 |
| 小鼠胚胎成纤维细胞–MEF | DF12+10%FBS+1%P/S 贴壁 |
| 小鼠胚胎成纤维细胞–MEF(丝裂霉素C处理) | DF12+10%FBS+1%P/S |
| 小鼠胚胎成骨细胞–MC3T3-E1 | MEMα+10%FBS+1%P/S 贴壁 |
| 小鼠脑微血管内皮细胞株–BEND.3 | DMEM(低碳酸氢钠)+10%FBS+1%P/S 贴壁 |
| 小鼠脑神经瘤细胞+LUC–neuro-2a+LUC(N2AN-2A) | MEM+10%FBS+1%P/S 贴壁 |
| 小鼠脑神经瘤细胞–neuro-2a=N2A | MEM+10%FBS+1%P/S 贴壁 |
| 小鼠脑神经胶质母细胞–G422来源不清晰不能出售 | DMEM+10%FBS+1%P/S |
| 小鼠卵巢上皮癌细胞–ID8 | DMEM+10%FBS+1%P/S +贴壁 |
| 小鼠颅顶前骨细胞亚克隆14–MC3T3-E1subclone-14 | MEMα(添加NaHCO31.5g-L+肌醇43.2mg-L+叶酸8.82mg-L+β-巯基乙醇7.8mg-L)+FBS 10%+P/S 1% |
| 小鼠淋巴瘤细胞–EL-4 | DMEM(低碳酸氢钠)+10%HS(或FBS)+1%P/S |
| 小鼠淋巴瘤细胞–L5178YTK+–clone(3.7.2C) | DMEM+10%FBS+1%P/S |
| 小鼠淋巴瘤细胞–YAC-1 | 1640+10%FBS+1%P/S 悬浮 |
| 小鼠淋巴结内皮细胞–SVEC4-10 | DMEM+10%FBS+1%P/S |
| 小鼠巨噬细胞–Ana-1 | 1640+10%FBS+1%P/S 悬浮 |
| 小鼠精原细胞系–GC-1spg | DMEM+10%FBS+1%P/S |
| 小鼠结肠癌细胞+LUC–CT26+LUC | 1640+10%FBS+1%P/S 贴壁 |
| 小鼠结肠癌细胞+LUC–MC38+LUC | DMEM+10%FBS+1%P/S |
| 小鼠结肠癌细胞–MC38 | DMEM+10%FBS+1%P/S |
| 小鼠结肠癌细胞–CT26 | 1640+10%FBS+1%P/S 贴壁 |
| 小鼠结肠癌细胞–CT26.WT | 1640+10%FBS+1%P/S 贴壁 |
| 小鼠胶质细胞瘤+LUC–GL261+LUC | DMEM+10%FBS+1%P/S |
| 小鼠胶质细胞瘤–GL261全新种子 | DMEM+10%FBS+1%P/S |
| 小鼠畸胎瘤细胞–P19 | MEMα+7.5%（NBS）+2.5%FBS+1%P/S |
| 小鼠黑色素瘤细胞荧光素酶标记–B16-F10+LUC | DMEM(低碳酸氢钠)+10%FBS+1%P/S |
| 小鼠黑色素瘤细胞–B16 | 1640（或IMDM）+10%FBS+1%P/S |
| 小鼠黑色素瘤细胞–B16B | 1640+10%（NBS）+1%P/S 贴壁 |
| 小鼠黑色素瘤细胞–B16-F10 | DMEM(低碳酸氢钠)+10%FBS+1%P/S 贴壁 |
| 小鼠海马神经元细胞–HT-22=HT22 | DMEM+10%FBS+1%P/S |
| 小鼠骨样细胞–MLO-Y4 | MEMa+5%FBS+5%（NBS）+1%P/S 鼠尾胶原包被 |
| 小鼠骨髓瘤细胞–Sp2-0 | 1640+10%FBS+1%P/S 圆形贴壁，传代时不可吹打！！！使其自然脱落 |
| 小鼠骨髓瘤细胞–Sp2-0-Ag14 | DMEM+10%FBS+1%P/S 悬浮不要用力吹打(生长时会沉到瓶底） |
| 小鼠骨髓基质细胞–OP9 | MEM-α+20%FBS+1%P/S 贴壁 |
| 小鼠骨肉瘤成骨细胞+LUC–K7M2.WT(K7M2-WT)+LUC | DMEM+10%FBS+1%P/S |
| 小鼠骨肉瘤成骨细胞–K7M2.WT(K7M2-wt) | DMEM+10%FBS+1%P/S |
| 小鼠睾丸间质细胞–TM3 | DF12+2.5%FBS+5%HS+1%P/S 贴壁 |
| 小鼠肝实质细胞–AML-12 | DF12+10%FBS+1%ITS（10ug-mL胰岛素；转铁蛋白5.5ug-mL;5ng-mL硒）+40ng-ml地塞米松+1%P/S |
| 小鼠肝癌细胞+LUC–HEPA1-6+LUC | 7 |
| 小鼠肝癌细胞–HEPA1-6 | DMEM(低碳酸氢钠)+10%FBS+1%丙酮酸纳+1%P/S 贴壁 |
| 小鼠肝癌细胞–H22 | 1640+10%FBS+1%P/S 悬浮 |
| 小鼠腹水瘤细胞–CCRFS-180II=S180 | 1640低碳酸氢钠)+glucose2.5g-L+SodiumPyruvate0.11g-L+10%FBS+1%P/S |
| 小鼠肺上皮细胞–MLE-12 | DF12+1%ITS（10ug-mL胰岛素；转铁蛋白5.5ug-mL;5ng-mL硒）+10nM氢化可的松+10nM雌二醇+10mMhepes+2mML-谷氨酰胺+2%FBS |
| 小鼠肺上皮细胞–TC-1 | 1640+10%FBS+1%P/S 贴壁 |
| 小鼠肺泡巨噬细胞–MH-S | 1640+10%FBS+0.05mM巯基乙醇（β-Me）半贴 |
| 小鼠肺癌细胞+LUC–LLC+LUC | DMEM+10%FBS+1%P/S |
| 小鼠肺癌细胞–LL-2（LLC1）=LLC | DMEM+10%FBS+1%P/S |
| 小鼠肥大细胞瘤细胞–P815 | 1640+10%FBS+1%P/S 悬浮，少量贴壁 |
| 小鼠单核巨噬细胞白血病细胞+GFP–RAW264.7+GFP | DMEM+10%FBS+1%P/S 贴壁，传代时密度要大，易分化 |
| 小鼠单核巨噬细胞白血病细胞–RAW264.7 | DMEM+10%FBS+1%P/S 贴壁，传代时密度要大，易分化 |
| 小鼠单核巨噬细胞–J774A.1 | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠成纤维细胞-结缔组织成纤维细胞–NCTC-clone-929（L929） | MEM+10%HS(HS)+1%P/S 贴壁 |
| 小鼠成肌细胞-骨骼肌–C2C12 | DMEM+10%FBS+1%P/S ,C2C12细胞生长较快，培养时细胞密度不可过高，否则容易分化 |
| 小鼠膀胱癌细胞–MB49 | DMEM+10%FBS+1%P/S |
| 小鼠白血病细胞–P388 | 1640+10%FBS+1%P/S |
| 小鼠肾腺癌细胞–RAG | MEM+10%FBS+1%P/S |
| 小鼠肾小管上皮细胞BSL-2–TCMK-1美国全新种子 | EMEM+10%FBS+1%P/S 贴壁很弱生长，温度敏感 |
| 小B细胞淋巴瘤细胞STR鉴定–A20=STR鉴定 | 1640+10%FBS+1%P/S |
| 小鼠睾丸间质细胞瘤细胞–MLTC-1生长缓慢 | 1640+10%FBS+1%P/S 贴壁 |
| 小鼠骨髓瘤淋巴母细胞-杂交瘤B淋巴细胞–IVA12 | DMEM+10%FBS+1%P/S 悬浮 |
| 小鼠骨髓淋巴母细胞–32D-clone3 | 1640+10%FBS+1ng-ml-IL3+1%P/S 贴壁+悬浮 |
| 小鼠T淋巴瘤细胞–E.G7-OVA | 1640+2mM 谷氨酰胺adjustedtocontain1.5g-Lsodiumbicarbonate,4.5g-Lglucose,10mMHEPESand1.0mMsodiumpyruvateandsupplementedwith0.05mM2-mercaptoethanoland0.4mg-mlG418,90%;FBS10%. |
| 小鼠肾系膜细胞–RMC进口 | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠血管内皮瘤细胞–EOMA | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠恶性乳腺癌细胞–Py230 | F12K+5%FBS+MITO+SerumExtender(Corning®#355006)0.1%+1%P/S |
| 小鼠恶性乳腺癌细胞–PY8119 | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠正常乳腺上皮细胞–NMuMG | DMEM+10%FBS+10ug-ml胰岛素+1%P/S 贴壁 |
| 小鼠前列腺癌细胞–MyC-CaP | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠血液细胞–WEHI-3 | 1640+10%FBS+1%P/S 悬浮 |
| 小鼠肺癌鳞癌细胞–KLN-205 | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠肝癌细胞–Hepa-1c1c7=STR | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠胚胎肝细胞–BNLCL.2 | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠Balbc脑内皮瘤–bEnd.5 | DMEM+10%FBS+1%P/S 贴壁 |
| 小鼠黑素瘤细胞–CloneM-3 | F12K+2.5%FBS+15%HS（HS）+1%P/S 贴壁 |
| 小鼠淋巴瘤细胞–BW5147 | 1640+10%FBS+1%P/S 贴壁+悬浮 |
| 小鼠杂交瘤细胞–LB3.1 | 1640+10%FBS+1%P/S 悬浮 |